rabbit anti mouse ng2  (Affinity Biosciences)

Journal: eLife

Article Title: Switching of RNA splicing regulators in immature neuroblasts during adult neurogenesis

doi: 10.7554/eLife.87083

Figure Lengend Snippet: ( A ) Population doublings (PD). Data were obtained from three independent experiments (n=8). ( B ) Clonogenicity assay. Results were obtained from two independent experiments. The number inside the bars indicated the number of microplate wells analyszed (mean ± SEM). ( C ) Differentiation assay. Representative images of immunofluorescence of freshly sorted iNB cells cultured in oligodendrocyte, astrocyte, or neuronal differentiation medium, and stained for NG2 and CNPase, GFAP and CD133 or βIII-Tubulin and doublecortin (DCX) expressions, respectively. *p<0.05, **p<0.01, ***p<0.001 (Mann-Whitney U-test). Scale bar: 20 µm. ( D ) EGFP-positive s-iNB were isolated from β−actin:eGFP mice and transplanted unilaterally at three injection points at proximity of the dSVZ/RMS of recipient C57Bl/6 J mice. Transplanted brains were analyszed 5 weeks later by immunostaining. ( a ) Detection of eGFP + cells in the granule cell layer and the external cell layer of the olfactory bulb of a mouse transplanted. (a1–a2) a2 high magnification of the inset (dotted line in a) showing eGFP + NeuN + cells (white arrows). ( b–c ) Detection of eGFP + GFAP + DCX + cells (yellow arrows) and eGFP +GFAP + (white arrows) in the dorsal ( b2 ) and lateral ( c2 ) SVZ. GCL:granule cell layer, EPL: external plexiform layer, GL: glomerular layer, lSVZ: lateral SVZ, dSVZ: dorsal SVZ, Scale bars = 40 µm or 100 µm.

Article Snippet: Antibody , Rabbit polyclonal anti-mouse NG2 , Merck Millipore , Cat#AB5320, RRID: AB_91789 , 1:100.

Techniques: Differentiation Assay, Immunofluorescence, Cell Culture, Staining, MANN-WHITNEY, Isolation, Injection, Immunostaining

Journal: eLife

Article Title: Switching of RNA splicing regulators in immature neuroblasts during adult neurogenesis

doi: 10.7554/eLife.87083

Figure Lengend Snippet: ( A ) Clonogenic potential of FACS-isolated eGFP + iNB and eGFP - iNB, and recombined eGFP + mNB isolated from DCX-CreERT2::CAG-floxed-eGFP mice, 7 days after the first injection of tamoxifen. ( B ) Population doublings (PD) of FACS-isolated eGFP + iNB and eGFP - iNB, eGFP + mNB cells and total iNB cells (regardless of eGFP status). No statistical difference was found in the growth rate of the different isolated populationss, Mann-Whitney U-test., (n=8) ( C ) Representative images of immunofluorescence of freshly sorted eGFP + iNB cells cultured in oligodendrocyte,astrocyte, or neuronal differentiation medium, and stained for NG2 and CNPase, GFAP and CD133, βIII-Tubulin and DCX, respectively. Scale: 10 µm. ( D ) Quantification of the percentage of eGFP + iNB among DAPI cells cultured in differentiation media that differentiate in either neurons (βIII-tubulin), oligodendrocytes (NG2) or astrocytes (GFAP).

Article Snippet: Antibody , Rabbit polyclonal anti-mouse NG2 , Merck Millipore , Cat#AB5320, RRID: AB_91789 , 1:100.

Techniques: Isolation, Injection, MANN-WHITNEY, Immunofluorescence, Cell Culture, Staining

Journal: eLife

Article Title: Switching of RNA splicing regulators in immature neuroblasts during adult neurogenesis

doi: 10.7554/eLife.87083

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit polyclonal anti-mouse NG2 , Merck Millipore , Cat#AB5320, RRID: AB_91789 , 1:100.

Techniques: Recombinant, Red Blood Cell Lysis, Software, Staining, Immunolabeling, FACS